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M94A3133.TXT
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Document 3133
DOCN M94A3133
TI Envelope V2 and V3 regions in circulating HIV-1 from patients with
SI-variants.
DT 9412
AU Telenti A; Grunow R; Deiss V; Bernasconi C; Matter L; von Overbeck J;
Univ. of Berne, Switzerland.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):146 (abstract no. PB0009). Unique
Identifier : AIDSLINE ICA10/94369442
AB BACKGROUND: SI phenotype may result from selection of a minority
species. To evaluate this possibility, we studied match serum and MT-2
culture supernatant. The V2 and V3 of gp120, known determinants of a SI
phenotype, were sequenced to establish how accurately cultured SI
isolates reflect the circulating species in vivo. M&M: Samples from 14
patients were evaluated by culture in MT-2 cells, and sequencing of env
from multiple clones obtained from culture supernatant and corresponding
sera. A SI genotype was defined as presence of elongation of the V2
hypervariable region and/or increase in positive charge of the V3 loop.
RESULTS: five groups were defined: TABULAR DATA, SEE ABSTRACT VOLUME.
CONCLUSIONS: 1) V2 and/or V3 SI-genotype was always present in culture
isolates exhibiting a SI phenotype. 2) The characteristics of V2 in
culture--but not V3--reflected what was found in serum in most patients.
3) Only 3 patients exhibited a fully homogeneous population of
SI-genotype variants in vivo and in vitro. Whether these patients
experience a different clinical evolution, and the possibility that MT-2
assays not only select minority strains, but allow the outgrowth of
variants not present in vivo need further assessment.
DE Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Cell Line Gene
Products, env/BIOSYNTHESIS/*GENETICS *Genes, env Genotype Human
HIV-1/*GENETICS/*ISOLATION & PURIF Phenotype Variation (Genetics)
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).